Signalling

Part:BBa_K4275000:Design

Designed by: Danni Huo   Group: iGEM22_GreatBay_SCIE   (2022-09-29)


Kluyveromyces marxianus alpha mating-factor secretion signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The sequence of K.marxianus alpha-mating factor secretion signal is derived from the genomic sequence of K.marxianus strain DMKU3-1042 (Genbank accesion number QGN17207.1)[1].

2. The Kex2 cleavage site (KR-EA) is highly-conserved between P.pastoris and K.marxianus, but S.cerevisiae and P.pastoris share an EAEA sequence[2], whilst K.marxianus has an EASA sequence. Other variations in the signal sequence are also emergent, for instance, the signal peptidase cleavage site, with a sequence of LA-AP in P.pastoris and IA-AP in K.marxianus.

Source

Kluyveromyces marxianus DMKU3-1042

References

1. “Mating Factor Alpha-1 [Kluyveromyces Marxianus] - Protein - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, https://www.ncbi.nlm.nih.gov/protein/QGN17207.1.

2. Chahal, Sabreen et al. “Structural characterization of the α-mating factor prepro-peptide for secretion of recombinant proteins in Pichia pastoris.” Gene vol. 598 (2017): 50-62. doi:10.1016/j.gene.2016.10.040